Baûng lieät keâ veà ñieàu kieän vaø moâi tröôøng

phaùt trieån cuûa vi khuaån Coronavirus cuûa SARS

 

Prepared for Internet by Vietnamese Missionaries in Asia

 

(WHO 6/05/2003) - Ngaøy 6/05/2003, Nhöõng nhaân vieân trong vieän nghieân cöùu cuûa Toå Chöùc Söùc Khoûe Theá Giôùi (WHO: World Health Organization) ñaõ ñöa ra moät baûng lieät keâ veà caùc ñieàu kieän vaø moâi tröôøng phaùt trieån cuûa vi khuaån Coronavirus cuûa SARS nhö sau:

Lab*

Substrate

Initial viral count log10PFU

Condition

Survival time

Method of testing viability

GVU

virus spiked in baby stool

1.00E+03

pH 6-7

3 hr

Virus isolation in cell culture

 

virus spiked in normal stool

7.50E+03

pH 8

6hr

Virus isolation in cell culture

 

virus in diarrheal stool

7.50E+03

pH 9

4days

Virus isolation in cell culture

QMH

stool

1.00E+03

Room Temperature

at least 2 days

Virus isolation in cell culture

 

urine

1.00E+03

Room Temperature

at least 24 hr

Virus isolation in cell culture

 

Virus culture medium+ 1% bovine serum

1.00E+03

on plastic surface in room temperature

at least 2 days

Virus isolation in cell culture

 

Virus culture medium+ 1% bovine serum

1.00E+04

30-37°C

at least 1hr

Virus isolation in cell culture

 

Virus culture medium+ 1% fetal calf serum

1.00E+04

56°C

degration of titre over time (10 000 infectious virus units in 15 min)

Virus isolation in cell culture

 

virus in Acetone, 10% Formaldehyde and Paraformaldehyde, 10% Clorox, 75%ethanol, 2% phenol

1.00E+06

Room Temperature

less than 5 min

Virus isolation in cell culture

NIID

Virus culture+ 2% bovine serum

1.00E+06

minus 80°C

at least 4 days

Virus isolation and RT-PCR

 

Virus culture+ 2% fetal calf serum

1.00E+06

4°C

at least 4 days

Virus isolation and RT-PCR

 

Virus culture+ 2% fetal calf serum

1.00E+06

37°C

less than 4 days

Virus isolation and RT-PCR

 

Virus culture+ 2% fetal calf serum

1.00E+05

56°C

less than 30min

 

UniM

Virus culture

1.00E+06

4°C

at least 21 days

Virus isolation

 

Virus culture

1.00E+06

minus 80°C

at least 21 days

Virus isolation

 

* Ghi chuù veà nhöõng teân vieát taét cuûa nhöõng phoøng thí nghieäm (Lab):

GVU: Government Virus Unit, Dept. of Health, Hong Kong, SAR China (Cô quan cuûa nhaø nöôùc nghieân cöùu veà vi khuaån, thuoäc UÛy Ban Söùc Khoûe, Hong Kong, SAR Trung Quoác)

QMH: Queen Mary Hospital, The University of Hong Kong, Hong Kong, SAR China (Beänh vieän Nöõ Hoaøng Mary, Ñaïi Hoïc Hong Kong, Hong Kong, SAR Trung Quoác)

NIID: National Institute of infectious Diseases, Tokyo, Japan (Vieän nghieân cöùu quoác gia veà vi truøng vaø dòch beänh, Tokyo, Nhaät Baûn)

UnivM: University Marburg, Germany (Ñaïi Hoïc Marburg, Ñöùc Quoác)

 

(Joseph Tröông)

 


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