Su phat trien cua SARS

Baûng lieät keâ veà ñieàu kieän vaø moâi tröôøng

phaùt trieån cuûa vi khuaån Coronavirus cuûa SARS

Prepared for Internet by Vietnamese Missionaries in Asia

(WHO 6/05/2003) - Ngaøy 6/05/2003, Nhöõng nhaân vieân trong vieän nghieân cöùu cuûa Toå Chöùc Söùc Khoûe Theá Giôùi (WHO: World Health Organization) ñaõ ñöa ra moät baûng lieät keâ veà caùc ñieàu kieän vaø moâi tröôøng phaùt trieån cuûa vi khuaån Coronavirus cuûa SARS nhö sau:

Lab*

Substrate

Initial viral count log_10PFU
_Condition _{Survival time} _{Method of testing viability}

_GVU _{virus spiked in baby stool} _1.00E+03 _{pH 6-7} _{3 hr} _{Virus isolation in cell culture}

_{virus spiked in normal stool} _7.50E+03 _{pH 8} _6hr _{Virus isolation in cell culture}

_{virus in diarrheal stool} _7.50E+03 _{pH 9} _4days _{Virus isolation in cell culture}

_QMH _stool _1.00E+03 _{Room Temperature} _{at least 2 days} _{Virus isolation in cell culture}

_urine _1.00E+03 _{Room Temperature} _{at least 24 hr} _{Virus isolation in cell culture}

_{Virus culture medium+ 1% bovine serum} _1.00E+03 _{on plastic surface in room temperature} _{at least 2 days} _{Virus isolation in cell culture}

_{Virus culture medium+ 1% bovine serum} _1.00E+04 _30-37^°C _{^{at least 1hr}} _{^{Virus isolation in cell culture}}

_{^{Virus culture medium+ 1% fetal calf
serum}} _^1.00E+04 _^56°C _{^{degration of titre over time (10 000
infectious virus units in 15 min)}} _{^{Virus isolation in cell culture}}

_{^{virus in Acetone, 10% Formaldehyde and
Paraformaldehyde, 10% Clorox, 75%ethanol, 2% phenol}} _^1.00E+06 _{^{Room Temperature}} _{^{less than 5 min}} _{^{Virus isolation in cell culture}}

_^NIID _{^{Virus culture+ 2% bovine serum}} _^1.00E+06 _{^{minus 80°C}} _{^{at least 4 days}} _{^{Virus isolation and RT-PCR}}

_{^{Virus culture+ 2% fetal calf serum}} _^1.00E+06 _^4°C _{^{at least 4 days}} _{^{Virus isolation and RT-PCR}}

_{^{Virus culture+ 2% fetal calf serum}} _^1.00E+06 _^37°C _{^{less than 4 days}} _{^{Virus isolation and RT-PCR}}

_{^{Virus culture+ 2% fetal calf serum}} _^1.00E+05 _^56°C _{^{less than 30min}}

_^UniM _{^{Virus culture}} _^1.00E+06 _^4°C _{^{at least 21 days}} _{^{Virus isolation}}

_{^{Virus culture}} _^1.00E+06 _{^{minus 80°C}} _{^{at least 21 days}} _{^{Virus isolation}}

* Ghi chuù veà nhöõng teân vieát taét cuûa nhöõng phoøng thí nghieäm (Lab):

GVU: Government Virus Unit, Dept. of Health, Hong Kong, SAR China (Cô quan cuûa nhaø nöôùc nghieân cöùu veà vi khuaån, thuoäc UÛy Ban Söùc Khoûe, Hong Kong, SAR Trung Quoác)

QMH: Queen Mary Hospital, The University of Hong Kong, Hong Kong, SAR China (Beänh vieän Nöõ Hoaøng Mary, Ñaïi Hoïc Hong Kong, Hong Kong, SAR Trung Quoác)

NIID: National Institute of infectious Diseases, Tokyo, Japan (Vieän nghieân cöùu quoác gia veà vi truøng vaø dòch beänh, Tokyo, Nhaät Baûn)

UnivM: University Marburg, Germany (Ñaïi Hoïc Marburg, Ñöùc Quoác)

(Joseph Tröông)

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Lab*	Substrate	Initial viral count log_10PFU	_Condition	_{Survival time}	_{Method of testing viability}
_GVU	_{virus spiked in baby stool}	_1.00E+03	_{pH 6-7}	_{3 hr}	_{Virus isolation in cell culture}
	_{virus spiked in normal stool}	_7.50E+03	_{pH 8}	_6hr	_{Virus isolation in cell culture}
	_{virus in diarrheal stool}	_7.50E+03	_{pH 9}	_4days	_{Virus isolation in cell culture}
_QMH	_stool	_1.00E+03	_{Room Temperature}	_{at least 2 days}	_{Virus isolation in cell culture}
	_urine	_1.00E+03	_{Room Temperature}	_{at least 24 hr}	_{Virus isolation in cell culture}
	_{Virus culture medium+ 1% bovine serum}	_1.00E+03	_{on plastic surface in room temperature}	_{at least 2 days}	_{Virus isolation in cell culture}
	_{Virus culture medium+ 1% bovine serum}	_1.00E+04	_30-37^°C	_{^{at least 1hr}}	_{^{Virus isolation in cell culture}}
	_{^{Virus culture medium+ 1% fetal calf serum}}	_^1.00E+04	_^56°C	_{^{degration of titre over time (10 000 infectious virus units in 15 min)}}	_{^{Virus isolation in cell culture}}
	_{^{virus in Acetone, 10% Formaldehyde and Paraformaldehyde, 10% Clorox, 75%ethanol, 2% phenol}}	_^1.00E+06	_{^{Room Temperature}}	_{^{less than 5 min}}	_{^{Virus isolation in cell culture}}
_^NIID	_{^{Virus culture+ 2% bovine serum}}	_^1.00E+06	_{^{minus 80°C}}	_{^{at least 4 days}}	_{^{Virus isolation and RT-PCR}}
	_{^{Virus culture+ 2% fetal calf serum}}	_^1.00E+06	_^4°C	_{^{at least 4 days}}	_{^{Virus isolation and RT-PCR}}
	_{^{Virus culture+ 2% fetal calf serum}}	_^1.00E+06	_^37°C	_{^{less than 4 days}}	_{^{Virus isolation and RT-PCR}}
	_{^{Virus culture+ 2% fetal calf serum}}	_^1.00E+05	_^56°C	_{^{less than 30min}}
_^UniM	_{^{Virus culture}}	_^1.00E+06	_^4°C	_{^{at least 21 days}}	_{^{Virus isolation}}
	_{^{Virus culture}}	_^1.00E+06	_{^{minus 80°C}}	_{^{at least 21 days}}	_{^{Virus isolation}}