Su phat trien cua SARS

Bảng liệt kê về điều kiện và môi trường

phát triển của vi khuẩn Coronavirus của SARS

Prepared for Internet by Vietnamese Missionaries in Asia

(WHO 6/05/2003) - Ngày 6/05/2003, Những nhân viên trong viện nghiên cứu của Tổ Chức Sức Khỏe Thế Giới (WHO: World Health Organization) đã đưa ra một bảng liệt kê về các điều kiện và môi trường phát triển của vi khuẩn Coronavirus của SARS như sau:

Lab*

Substrate

Initial viral count log_10PFU
_Condition _{Survival time} _{Method of testing viability}

_GVU _{virus spiked in baby stool} _1.00E+03 _{pH 6-7} _{3 hr} _{Virus isolation in cell culture}

_{virus spiked in normal stool} _7.50E+03 _{pH 8} _6hr _{Virus isolation in cell culture}

_{virus in diarrheal stool} _7.50E+03 _{pH 9} _4days _{Virus isolation in cell culture}

_QMH _stool _1.00E+03 _{Room Temperature} _{at least 2 days} _{Virus isolation in cell culture}

_urine _1.00E+03 _{Room Temperature} _{at least 24 hr} _{Virus isolation in cell culture}

_{Virus culture medium+ 1% bovine serum} _1.00E+03 _{on plastic surface in room temperature} _{at least 2 days} _{Virus isolation in cell culture}

_{Virus culture medium+ 1% bovine serum} _1.00E+04 _30-37^�C _{^{at least 1hr}} _{^{Virus isolation in cell culture}}

_{^{Virus culture medium+ 1% fetal calf
serum}} _^1.00E+04 _^56�C _{^{degration of titre over time (10 000
infectious virus units in 15 min)}} _{^{Virus isolation in cell culture}}

_{^{virus in Acetone, 10% Formaldehyde and
Paraformaldehyde, 10% Clorox, 75%ethanol, 2% phenol}} _^1.00E+06 _{^{Room Temperature}} _{^{less than 5 min}} _{^{Virus isolation in cell culture}}

_^NIID _{^{Virus culture+ 2% bovine serum}} _^1.00E+06 _{^{minus 80�C}} _{^{at least 4 days}} _{^{Virus isolation and RT-PCR}}

_{^{Virus culture+ 2% fetal calf serum}} _^1.00E+06 _^4�C _{^{at least 4 days}} _{^{Virus isolation and RT-PCR}}

_{^{Virus culture+ 2% fetal calf serum}} _^1.00E+06 _^37�C _{^{less than 4 days}} _{^{Virus isolation and RT-PCR}}

_{^{Virus culture+ 2% fetal calf serum}} _^1.00E+05 _^56�C _{^{less than 30min}}

_^UniM _{^{Virus culture}} _^1.00E+06 _^4�C _{^{at least 21 days}} _{^{Virus isolation}}

_{^{Virus culture}} _^1.00E+06 _{^{minus 80�C}} _{^{at least 21 days}} _{^{Virus isolation}}

* Ghi chú về những tên viết tắt của những phòng thí nghiệm (Lab):

GVU: Government Virus Unit, Dept. of Health, Hong Kong, SAR China (Cơ quan của nhà nước nghiên cứu về vi khuẩn, thuộc Ủy Ban Sức Khỏe, Hong Kong, SAR Trung Quốc)

QMH: Queen Mary Hospital, The University of Hong Kong, Hong Kong, SAR China (Bệnh viện Nữ Hoàng Mary, Ðại Học Hong Kong, Hong Kong, SAR Trung Quốc)

NIID: National Institute of infectious Diseases, Tokyo, Japan (Viện nghiên cứu quốc gia về vi trùng và dịch bệnh, Tokyo, Nhật Bản)

UnivM: University Marburg, Germany (Ðại Học Marburg, Ðức Quốc)

(Joseph Trương)

Back to Vietnamese Missionaries in Asia Home Page

Lab*	Substrate	Initial viral count log_10PFU	_Condition	_{Survival time}	_{Method of testing viability}
_GVU	_{virus spiked in baby stool}	_1.00E+03	_{pH 6-7}	_{3 hr}	_{Virus isolation in cell culture}
	_{virus spiked in normal stool}	_7.50E+03	_{pH 8}	_6hr	_{Virus isolation in cell culture}
	_{virus in diarrheal stool}	_7.50E+03	_{pH 9}	_4days	_{Virus isolation in cell culture}
_QMH	_stool	_1.00E+03	_{Room Temperature}	_{at least 2 days}	_{Virus isolation in cell culture}
	_urine	_1.00E+03	_{Room Temperature}	_{at least 24 hr}	_{Virus isolation in cell culture}
	_{Virus culture medium+ 1% bovine serum}	_1.00E+03	_{on plastic surface in room temperature}	_{at least 2 days}	_{Virus isolation in cell culture}
	_{Virus culture medium+ 1% bovine serum}	_1.00E+04	_30-37^�C	_{^{at least 1hr}}	_{^{Virus isolation in cell culture}}
	_{^{Virus culture medium+ 1% fetal calf serum}}	_^1.00E+04	_^56�C	_{^{degration of titre over time (10 000 infectious virus units in 15 min)}}	_{^{Virus isolation in cell culture}}
	_{^{virus in Acetone, 10% Formaldehyde and Paraformaldehyde, 10% Clorox, 75%ethanol, 2% phenol}}	_^1.00E+06	_{^{Room Temperature}}	_{^{less than 5 min}}	_{^{Virus isolation in cell culture}}
_^NIID	_{^{Virus culture+ 2% bovine serum}}	_^1.00E+06	_{^{minus 80�C}}	_{^{at least 4 days}}	_{^{Virus isolation and RT-PCR}}
	_{^{Virus culture+ 2% fetal calf serum}}	_^1.00E+06	_^4�C	_{^{at least 4 days}}	_{^{Virus isolation and RT-PCR}}
	_{^{Virus culture+ 2% fetal calf serum}}	_^1.00E+06	_^37�C	_{^{less than 4 days}}	_{^{Virus isolation and RT-PCR}}
	_{^{Virus culture+ 2% fetal calf serum}}	_^1.00E+05	_^56�C	_{^{less than 30min}}
_^UniM	_{^{Virus culture}}	_^1.00E+06	_^4�C	_{^{at least 21 days}}	_{^{Virus isolation}}
	_{^{Virus culture}}	_^1.00E+06	_{^{minus 80�C}}	_{^{at least 21 days}}	_{^{Virus isolation}}